SubtiBank SubtiBank
relA [2020-04-06 11:01:21]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

relA [2020-04-06 11:01:21]

GTP pyrophosphokinase (stringent response)
Locus
BSU_27600
Isoelectric point
6.96
Molecular weight
84.65 kDa
Protein length
734 aa Sequence Blast
Gene length
2205 bp Sequence Blast
Function
synthesis and degradation of (p)ppGpp, triggering the stringent response
Product
GTP pyrophosphokinase
Essential
no
E.C.
2.7.6.5
Synonyms

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
2,820,529 → 2,822,733

Phenotypes of a mutant

  • requirement for valine PubMed
  • a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed
  • a relA sasA sasB triple mutant acquires suppressor mutations in guaA, guaB, gmk or inactivation of codY PubMed
  • mutation in relA results in increased motility and chaining via elevated SigD level PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + GTP --> AMP + guanosine 3'-diphosphate 5'-triphosphate (according to UniProt)
  • Protein family

  • RelA/SpoT family (with SasA and SasB, according to UniProt)
  • Domains

  • N-terminal ppGpp hydrolase domain (HD domain) (aa 31-180) PubMed
  • central ppGpp synthetase domain PubMed
  • TGS domain (aa 392-453) (according to UniProt)
  • C-terminal ACT domain (aa 660-734) (responsible for fine-tuning of activation at the ribosome) PubMed
  • Effectors of protein activity

  • (p)ppGpp synthesis is activated by uncharged tRNAs in a ribosome-dependent manner
  • the interaction with ComGA inhibits the hydrolysis of ppGpp PubMed
  • heat stress triggers (p)ppGpp synthesis PubMed
  • the presence of immature tRNAs due to depletion of [RNase P] or RNase Z triggers (p)ppGpp synthesis by RelA PubMed
  • Structure

  • 5IQR (from E. coli, bound to the ribosome, 38.8% identity)
  • 1VJ7 (N-terminal catalytic fragment, from Streptococcus equisimilis, 60% identity) PubMed
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • relA mutant PubMed - note that relA mutants are prone to suppressor mutations in the sasA or sasB loci PubMed
  • GP2066 (sasA sasB relA::mls), available in Jörg Stülke's lab
  • BKE27600 (ΔrelA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGAATCACCTTTTTTAA, downstream forward: _UP4_TAAAGGGGTTAGAAAAGAGA
  • BKK27600 (ΔrelA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGAATCACCTTTTTTAA, downstream forward: _UP4_TAAAGGGGTTAGAAAAGAGA
  • Labs working on this gene/protein

  • Mohamed Marahiel, Marburg University, Germany homepage
  • Jue D Wang, University of Wisconsin–Madison ResearchGate-Profile
  • References

    Reviews

    Loading

    Original publications

    Loading